Nine centuries ago, people gathered at what is today recognized as the Etowah Indian Mounds site, located roughly 40 miles northwest of Atlanta, Georgia, for enormous community feasts. They ate, drank, and eventually discarded broken pottery into pits that would become part of the foundations of the ancient city’s first monumental mound.
Now, traces hidden inside two pieces of that discarded pottery have revealed something archaeologists never expected to find so far from the tropics: ancient cacao DNA.
Researchers say the discovery provides the first direct evidence that cacao, the plant used to make chocolate, reached the Mississippian civilization of the eastern United States roughly 800 to 900 years ago.
Because cacao cannot grow naturally in Georgia, its presence also points to surprisingly extensive networks capable of moving exotic goods across long stretches of pre-Columbian North America. The findings were published in PLOS One.
“This study presents the first evidence that cacao consumption was part of the Mississippian world at roughly the same time as it appeared at Chaco,” researchers write. “This discovery at Etowah asks us to broaden our understanding of the networks that distributed cacao throughout the Americas before European colonization.”
Chocolate has a deep history in the Americas. Archaeological evidence indicates cacao was being consumed in South America at least 5,000 years ago and later became culturally important throughout Central America.
More surprising was a 2009 discovery indicating that people at Chaco Canyon in modern New Mexico drank cacao between roughly 1050 and 1350 CE. Since cacao only grows in tropical climates, the finding meant long-distance exchange networks had carried the plant hundreds of miles northward.
Whether cacao traveled even farther into what is now the eastern United States remained unclear.
However, Etowah offered an intriguing place to look. Located in modern Georgia, the Indigenous city eventually became one of the most important centers of Mississippian civilization. The pottery examined in the new study came from feasting deposits dating to the 11th and 12th centuries, early in Etowah’s history.
Researchers had previously analyzed residues from 31 Etowah pottery fragments. Twenty-one contained at least two chemical compounds associated with caffeinated beverages, while three contained all four compounds the scientists examined.
Yaupon holly, a native plant used to produce the caffeinated beverage historically known as Black Drink, contains several of the same chemical biomarkers as cacao. Researchers, therefore, concluded that the Etowah vessels most likely contained yaupon rather than cacao.
However, this time, scientists went looking for cacao itself.
Researchers subjected samples from three pottery fragments to ancient DNA analysis, producing more than 502 million useful paired sequences before using successive filters to identify genetic material specific to Theobroma cacao.
Two pottery samples cleared the researchers’ threshold for identifying cacao.
Importantly, the genetic material also displayed telltale characteristics of ancient DNA. The fragments averaged only 84 base pairs long, consistent with the degradation expected after centuries of decay. The researchers also employed strict contamination controls and bioinformatic filtering to distinguish cacao from other organisms present in the samples.
The evidence does not establish exactly how cacao reached Georgia. Preliminary genetic comparisons linked one sample most closely to Amelonado cacao and another to Curaray, both of which also showed similarities to Marañon cacao. Those lineages originated in the Amazon basin, including parts of modern Ecuador, Peru, and Brazil, but also spread into Central America long before cacao appeared at Etowah.
Cacao may have traveled from Central America through long-distance exchange networks or passed indirectly through communities in the American Southwest before reaching the Eastern Woodlands. However, too few genetic markers survived to pinpoint its immediate source, and researchers say no direct archaeological evidence currently establishes either route.
Perhaps more intriguing is where researchers found the cacao.
The pottery came from pits packed with animal bones, ceramics, and organic material interpreted as the remains of communal feasting associated with the construction of Etowah’s first platform mound. Researchers suggest cacao was more than an exotic luxury and could have played a role in ceremonies intended to reinforce social bonds during the city’s formative period.
The discovery also raises the possibility that previously detected chemical residues at other Mississippian sites, including Cahokia, could contain cacao that was mistaken for locally available yaupon. Confirming that possibility will require additional ancient DNA testing.
“These results ask us to reopen lines of research long closed, especially regarding contacts between the impressive civilizations of Central and South America and the great Mississippian civilization of the Eastern Woodlands,” researchers write. “More broadly, our results suggest that it is time to explore the possibility that cacao was distributed more widely in the precontact Americas than we have understood.”
Study: King, A., Powis, T.G., Argout, X., Vignes, H., Utge, J., Rhoné, B., et al. “Cacao in the Mississippian World: Archaeogenomic evidence for T. cacao consumption at the Etowah Site, Georgia.” PLOS One 21(8):(2026).
Tim McMillan is a retired law enforcement executive, investigative reporter and co-founder of The Debrief. His writing typically focuses on defense, national security, the Intelligence Community and topics related to psychology. You can follow Tim on Twitter: @LtTimMcMillan. Tim can be reached by email: tim@thedebrief.org or through encrypted email: LtTimMcMillan@protonmail.com